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We employed the movement cytometry technique of Pulse Shape Evaluation to purify cells expressing mutant Httex1-mCherry into these with inclusions (i) from these without inclusions (ni; dispersed uniformly in the cytosol) at matched median expression levels ( Moily et al., 2017 ; Ramdzan et al., 2012 ). This technique enabled us to assess how the aggregation state of mutant Httex1 (97Q ni and i) affected proteome solubility in comparison with a wild-kind state (25Q ni – notice that 25Q does not form aggregates) ( Fig 2A and Fig S1 ).
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