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For the Hsp70 and Hsp90 exercise inhibition experiments, Neuro2a cells have been cultured in medium supplemented with 20 µM Ver155008 (Sigma) for 18 hours (h) or 800 µM novobiocin (Sigma) for 6 h. To dam the proteolytic exercise of 26S proteasome advanced, cells had been burdened in medium supplemented with 5 µM MG132 (Sigma) for 18 h. To induce oxidative stress, cells had been treated with 500 µM sodium arsenite (Sigma) for 1 h. To induce ER stress, cells have been handled with 5 µM tunicamycin (Sigma) for 18 h. For Httex1 stress, Neuro2a cells have been transiently transfected with the vectors using Lipofectamine 2000 reagent (Invitrogen).

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