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We employed the movement cytometry method of Pulse Form Analysis to purify cells expressing mutant Httex1-mCherry into those with inclusions (i) from those with out inclusions (ni; dispersed uniformly within the cytosol) at matched median expression ranges ( Moily et al., 2017 ; Ramdzan et al., 2012 ). This strategy enabled us to evaluate how the aggregation state of mutant Httex1 (97Q ni and i) affected proteome solubility in comparison with a wild-sort state (25Q ni – notice that 25Q does not form aggregates) ( Fig 2A and Fig S1 ).