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Welcome to the Division of New Media Expertise (NMT), which undertakes interdisciplinary analysis on information acquisition and administration, develops media monitoring and enterprise intelligence functions, and investigates the potential of latest media for interactive advertising and marketing and constructing digital communities.
For the Hsp70 and Hsp90 exercise inhibition experiments, Neuro2a cells have been cultured in medium supplemented with 20 ÂµM Ver155008 (Sigma) for 18 hours (h) or 800 ÂµM novobiocin (Sigma) for 6 h. To dam the proteolytic exercise of 26S proteasome advanced, cells had been burdened in medium supplemented with 5 ÂµM MG132 (Sigma) for 18 h. To induce oxidative stress, cells had been treated with 500 ÂµM sodium arsenite (Sigma) for 1 h. To induce ER stress, cells have been handled with 5 ÂµM tunicamycin (Sigma) for 18 h. For Httex1 stress, Neuro2a cells have been transiently transfected with the vectors using Lipofectamine 2000 reagent (Invitrogen).
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